![]() This peptide was designated frog GH-releasing peptide. Thus, the amphibian hypothalamus was revealed to contain a novel functional RFa peptide that stimulates GH release. It was ascertained that the peptide was also effective in elevating the circulating GH level when administered systemically. Aya Endo (born Februin Yamagata Prefecture, Japan) is one of. This peptide did not have any appreciable effect on the secretion of PRL and gonadotropins. ![]() This RFa peptide stimulated, in a dose-related way, the release of GH from cultured pituitary cells, its threshold concentration ranging between 10(-9) and 10(-8) M. The cell bodies and terminals containing this peptide were localized immunohistochemically in the suprachiasmatic nucleus and median eminence, respectively. Endou Aya is most famous for voicing Takara Miyuki in Lucky. She is known for Resonance of Fate (2010), Project X Zone (2012) and Guilty Crown (2011). Endou Aya has announced in the last message she posted on her official blog that she has gotten married. The isolated peptide was a novel RFa peptide and shown to have the sequence Ser-Leu-Lys-Pro-Ala-Ala-Asn-Leu-Pro-Leu-Arg-Phe-NH2. Aya Endô was born on Februin Yamagata, Japan. Immunoreactive fractions were subjected to further cation exchange and reversed-phase HPLC purification. RFa immunoreactivity was measured in the eluted fractions by a dot immunoblot assay employing an antiserum raised against RFa. An acid extract of bullfrog hypothalami was passed through C-18 reversed-phase cartridges, and then the retained material was subjected to HPLC, initially using a C-18 reversed-phase column. ![]() In this study, we sought to identify an amphibian hypothalamic RFa peptide that may regulate secretion of hormones by the anterior pituitary gland. Neuropeptides similar to the molluscan cardioexcitatory Phe-Met-Arg-Phe-NH2 have been identified in several vertebrates and characterized by the RFa motif at their C terminus (RFa peptides).
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